Isolation, NMR Characterization, and Bioactivity of a Flavonoid Triglycoside from <i>Anthyllis henoniana</i> Stems: Antioxidant and Antiproliferative Effects on MDA-MB-231 Breast Cancer Cells
Amani Ayachi,
Guillaume Boy,
Sonda Samet,
Nathan Téné,
Bouthaina Bouzayani,
Michel Treilhou,
Raoudha Mezghani-Jarraya,
Arnaud Billet
Affiliations
Amani Ayachi
Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, Tunisia
Guillaume Boy
Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France
Sonda Samet
Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, Tunisia
Nathan Téné
Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France
Bouthaina Bouzayani
Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, Tunisia
Michel Treilhou
Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France
Raoudha Mezghani-Jarraya
Laboratory of Organic Chemistry LR17ES08, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, Tunisia
Arnaud Billet
Equipe BTSB-EA 7417, Institut National Universitaire Jean-François Champollion, Université de Toulouse, Place de Verdun, 81012 Albi, France
Plant extracts are considered as a large source of active biomolecules, especially in phytosanitary and pharmacological fields. Anthyllis henoniana is a woody Saharan plant located in the big desert of North Africa. Our previous research paper proved the richness of the methanol extract obtained from the stems in flavonoids and phenolic compounds as well as its remarkable antioxidant activity. In this research, we started by investigating the phytochemical composition of the methanol extract using high performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC–MS/MS). Among the 41 compounds identified, we isolated and characterized (structurally and functionally) the most abundant product, a flavonoid triglycoside (AA770) not previously described in this species. This compound, which presents no cytotoxic activity, exhibits an interesting cellular antioxidant effect by reducing reactive oxygen species (ROS) generation, and an antiproliferative action on breast cancer cells. This study provides a preliminary investigation into the pharmacological potential of the natural compound AA770, isolated and identified from Anthyllis henoniana for the first time.
