Pollen Morphology and Sucrose Giving on Stigma Relation with Incopability of Sweetpotato [Ipomoea Batatas (L.) L.]

Abstract

This research aimed to increase the success rate of self-pollination in sweetpotato through sucrose treatment on stigma and pollen nuclei check. Various concentration of sucrose was applied on the stigma. Prior to the crosses, the stigma was excised and lubricated with sucrose. Pedicel was lubricated with 100 ppm of 2,4 D in lanolin. The result showed that the stigma of both varieties was longer than the anther. Papua Solosa showed 94,7% anther dehiscence and 70, 25% pollen viability; whereas MSU 03028-10 showed 87, 3% anther dehiscence and 87, 84% pollen viability. Both of these varieties showed receptive stigma. Sucrose level in compatible pollination was lower than in incompatible pollination. Sugar in 50 ppm concentration can increase the formation of fruit in self-pollination of Papua Solosa varieties from 0% to 70%; 0% to 60% in self-pollination of MSU 03028-10; and 10% to 90% of Papua Solosa><MSU 03028-10. The pollen nuclei of both of varieties were trinucleate. Sweetpotato incompatibility system was sporophytic self-incompatibility type that can be fixed by sucrose treatment using 50 ppm concentration, stigma excision, and 100 ppm of 2,4D in lanolin.

Keywords

• pollen morphology
• sucrose giving
• incompatibility of sweet potato