A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

Jerome Jatzlau; Wiktor Burdzinski; Michael Trumpp; Leon Obendorf; Kilian Roßmann; Katharina Ravn; Marko Hyvönen; Francesca Bottanelli; Johannes Broichhagen; Petra Knaus

doi:10.1038/s42003-022-04388-4

Communications Biology (Jan 2023)

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

Jerome Jatzlau,
Wiktor Burdzinski,
Michael Trumpp,
Leon Obendorf,
Kilian Roßmann,
Katharina Ravn,
Marko Hyvönen,
Francesca Bottanelli,
Johannes Broichhagen,
Petra Knaus

Affiliations

Jerome Jatzlau: Institute of Chemistry and Biochemistry - Biochemistry
Wiktor Burdzinski: Institute of Chemistry and Biochemistry - Biochemistry
Michael Trumpp: Institute of Chemistry and Biochemistry - Biochemistry
Leon Obendorf: Institute of Chemistry and Biochemistry - Biochemistry
Kilian Roßmann: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Katharina Ravn: Department of Biochemistry, University of Cambridge
Marko Hyvönen: Department of Biochemistry, University of Cambridge
Francesca Bottanelli: Institute of Chemistry and Biochemistry - Biochemistry
Johannes Broichhagen: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Petra Knaus: Institute of Chemistry and Biochemistry - Biochemistry

DOI: https://doi.org/10.1038/s42003-022-04388-4
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 15

Abstract

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A TGFβ/BMP receptor library with Halo- and SNAP-tags is generated and with the application of labeled growth factor ligands, a ligand surface binding assay is developed, allowing assessment of preferential ligand-receptor binding in cells.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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