OncoTargets and Therapy (Jan 2020)

Up-Regulated CCDC34 Contributes to the Proliferation and Metastasis of Hepatocellular Carcinoma

  • Lin Z,
  • Qu S,
  • Peng W,
  • Yang P,
  • Zhang R,
  • Zhang P,
  • Guo D,
  • Du J,
  • Wu W,
  • Tao K,
  • Wang J

Journal volume & issue
Vol. Volume 13
pp. 51 – 60

Abstract

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Zhibin Lin,* Shibin Qu,* Wei Peng,* Peijun Yang, Ruohan Zhang, Pengcheng Zhang, Dongnan Guo, Jianbing Du, Wenlong Wu, Kaishan Tao, Jianlin Wang Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an, 710032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jianlin Wang; Kaishan TaoDepartment of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, 127 West Changle Street, Xi’an, Shaanxi 710032, People’s Republic of ChinaTel +86 29 8477 5259Fax +86 29 8477 1595Email [email protected]; [email protected]: Coiled-coil domain-containing protein 34 (CCDC34), which belongs to the CCDCs family, has been recently reported to be up-regulated in various kinds of tumors. However, its role in the development of hepatocellular carcinoma (HCC) still remains unclear.Materials and methods: In this study, real-time polymerase chain reaction (RT-PCR) and Western blot analysis were performed to measure the mRNA and protein levels of CCDC34 in clinical samples. Kaplan-Meier method was used to analyze the relationship between CCDC34 and the prognosis in HCC patients. CCK-8 and colony formation assays were conducted to investigate CCDC34’s effect on the cell proliferation, and Transwell assays were used to detect CCDC34’s effect on the cell metastasis. Moreover, subcutaneous xenograft tumor model and lung metastasis model were applied to confirm the impact of CCDC34 on the HCC development. Lastly, RNA sequencing and Western blot analysis were performed to probe the underlying mechanism of CCDC34’s effect on HCC.Results: CCDC34 was significantly induced in HCC tissues, and the overexpression of CCDC34 predicted the poor outcomes among HCC patients. It was verified by the in vitro and in vivo experiments that CCDC34-knockdown potently inhibited the proliferation and metastasis of HCC cells. Subsequent results indicated that CCDC34 inhibition can affect the activation of protein kinase B (PKB or AKT) as well as epithelial-mesenchymal transition (EMT) process.Conclusion: CCDC34 is significantly associated with HCC. It will become a promising prognostic biomarker and therapeutic target against HCC.Keywords: CCDC34, HCC, proliferation, EMT, PI3K/AKT, CCND1

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