In this research, we proposed a potentiometric sensor based on copper doped zinc oxide (CZO) films to detect glucose. Silver nanowires were used to improve the sensor’s average sensitivity, and we used the low power consumption instrumentation amplifier (UGFPCIA) designed by our research group to measure the sensing characteristics of the sensor. It was proved that the sensor performs better when using this system. In order to observe the stability of the sensor, we also studied the influence of two kinds of non-ideal effects on the sensor, such as the drift effect and the hysteresis effect. For this reason, we chose to combine the calibration readout circuit with the voltage-time (V-T) measurement system to optimize the measurement environment and successfully reduced the instability of the sensor. The drift rate was reduced by about 51.1%, and the hysteresis rate was reduced by 13% and 28% at different measurement cycles. In addition, the characteristics of the sensor under dynamic conditions were also investigated, and it was found that the sensor has an average sensitivity of 13.71 mV/mM and the linearity of 0.998 at a flow rate of 5.6 μL/min.
