Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul (Nov 2017)
Detection of bla TEM, bla CTX-M and bla SHV in Salmonella Species Isolated from Children with Acute Infectious Diarrhea by Multiplex-PCR and Their Antibiotic Resistance Profile
Abstract
BACKGROUND AND OBJECTIVE: Treatment for Salmonella gastroenteritis is important in children and people with immune system weakness. The aim of this study was to investigate the presence of bla SHV, bla TEM and bla CTX-M genes in antibiotic resistance to isolated Salmonella strains from children with acute bacterial diarrhea and to determine their resistance pattern. METHODS: In this descriptive cross-sectional study, 300 stool specimens from children with diarrhea referred to the Tehran Medical Center Hospital were collected. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for determination of β-lactamase genes by specific oligonucleotides primers. Data were analyzed by SPSS software version 17 and descriptive statistics. FINDINGS: Of the 300 stool samples collected, 18 (6%) of Salmonella were identified, of which 11 (61.1%) were salmonella typhi, 5 (27.7%) were Salmonella enteritidis and 2 (11.1%) Salmonella typhimorium cases. Resistance to isolates showed that the highest and lowest resistance was related to imipenem, ceftriaxone (100%) and onloxacin (54.5%) respectively. The results of the molecular analysis indicated that 7 strains (38.8%) CTX-M and 8 isolates (44.4%) had TEM genes respectively, while 2 strains (11.1%) contained the SHV gene. CONCLUSION: accurate detection and fast identification of Salmonella producing ESBLs is important from the source of infection such as, foods, animals and its products and carriers.
