A Novel Method to Image Macropinocytosis in Vivo

Lunhao Chen; Lunhao Chen; Daxiao Cheng; Jiachen Chu; Jiachen Chu; Ting Zhang; Zhuoer Dong; Huifang Lou; Liya Zhu; Yijun Liu

doi:10.3389/fnins.2018.00324

Frontiers in Neuroscience (May 2018)

A Novel Method to Image Macropinocytosis in Vivo

Lunhao Chen,
Lunhao Chen,
Daxiao Cheng,
Jiachen Chu,
Jiachen Chu,
Ting Zhang,
Zhuoer Dong,
Huifang Lou,
Liya Zhu,
Yijun Liu

Affiliations

Lunhao Chen: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Lunhao Chen: Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Daxiao Cheng: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Jiachen Chu: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Jiachen Chu: Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States
Ting Zhang: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Zhuoer Dong: Middle School Attached to Northwestern Polytechnical University, Xi'an, China
Huifang Lou: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Liya Zhu: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Yijun Liu: Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China

DOI: https://doi.org/10.3389/fnins.2018.00324
Journal volume & issue: Vol. 12

Abstract

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Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we monitored the generation of dextran-positive macropinosomes and subsequent intracellular events. Our protocol provides a continent and reliable way for investigating macropinocytosis and its underlying mechanisms, especially when combined with genetic strategies.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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Abstract

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