Fecal transplant from vaginally seeded infants decreases intraabdominal adiposity in mice
Sivaranjani Namasivayam,
Curtis Tilves,
Hua Ding,
Shaoguang Wu,
Jada C Domingue,
Camilo Ruiz-Bedoya,
Ankit Shah,
Eric Bohrnsen,
Benjamin Schwarz,
Mickayla Bacorn,
Qing Chen,
Shira Levy,
Maria Gloria Dominguez Bello,
Sanjay K Jain,
Cynthia L Sears,
Noel T Mueller,
Suchitra K Hourigan
Affiliations
Sivaranjani Namasivayam
Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Curtis Tilves
Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA
Hua Ding
Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Shaoguang Wu
Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Jada C Domingue
Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Camilo Ruiz-Bedoya
Center for Infection and Inflammation Imaging Research, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Ankit Shah
Inova Health System, Inova Women’s Hospital, Falls Church, VA, USA
Eric Bohrnsen
Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases; National Institutes of Health, Hamilton, MT, USA
Benjamin Schwarz
Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases; National Institutes of Health, Hamilton, MT, USA
Mickayla Bacorn
Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Qing Chen
Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Shira Levy
Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Maria Gloria Dominguez Bello
Departments of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA
Sanjay K Jain
Center for Infection and Inflammation Imaging Research, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Cynthia L Sears
Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Noel T Mueller
Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA
Suchitra K Hourigan
Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Exposing C-section infants to the maternal vaginal microbiome, coined “vaginal seeding”, partially restores microbial colonization. However, whether vaginal seeding decreases metabolic disease risk is unknown. Therefore, we assessed the effect of vaginal seeding of human infants on adiposity in a murine model. Germ-free mice were colonized with transitional stool from human infants who received vaginal seeding or control (placebo) seeding in a double-blind randomized trial. There was a reduction in intraabdominal adipose tissue (IAAT) volume in male mice that received stool from vaginally seeded infants compared to control infants. Higher levels of isoleucine and lower levels of nucleic acid metabolites were observed in controls and correlated with increased IAAT. This suggests that early changes in the gut microbiome and metabolome caused by vaginal seeding have a positive impact on metabolic health.
