A Novel Benzothiazole-Based Fluorescent AIE Probe for the Detection of Hydrogen Peroxide in Living Cells
Dezhi Shi,
Yulong Yang,
Luan Tong,
Likang Zhang,
Fengqing Yang,
Jiali Tao,
Mingxia Zhao
Affiliations
Dezhi Shi
The Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, China
Yulong Yang
Department of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
Luan Tong
Department of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
Likang Zhang
Department of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
Fengqing Yang
The Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, China
Jiali Tao
The Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, China
Mingxia Zhao
The Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, China
A benzothiazole-based derivative aggregation-induced emission (AIE) fluorescent ‘turn-on’ probe named 2-(2-((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)phenyl)benzo[d]thiazole (probe BT-BO) was developed and synthesized successfully for detecting hydrogen peroxide (H2O2) in living cells. The synthesis method of probe BT-BO is facile. Probe BT-BO demonstrates a well-resolved emission peak at 604 nm and the ability to prevent the interference of reactive oxygen species (ROS), various metal ions and anion ions, and good sensitivity. Additionally, the probe boasts impressive pH range versatility, a fast response time to H2O2 and low cytotoxicity. Finally, probe BT-BO was applied successfully to image A549 and Hep G2 cells to monitor both exogenous and endogenous H2O2.
