Frontiers in Molecular Biosciences (Dec 2021)

Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer

  • Haiyan Chen,
  • Qi Sun,
  • Cangang Zhang,
  • Junjun She,
  • Shuai Cao,
  • Meng Cao,
  • Nana Zhang,
  • Ayarick Vivian Adiila,
  • Jinjin Zhong,
  • Chengyun Yao,
  • Yili Wang,
  • Hongping Xia,
  • Hongping Xia,
  • Linhua Lan

DOI
https://doi.org/10.3389/fmolb.2021.756085
Journal volume & issue
Vol. 8

Abstract

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Gastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, development, and metastasis. Twenty-two kinds of immune cells were calculated by CIBERSORT from Gene Expression Omnibus (GEO) database. Subsequently, higher infiltration of macrophages M0 was discovered in GC compared with normal tissues. WGCNA was utilized to construct the network and then identify key modules and genes related to macrophages in TCGA. Finally, 18 hub genes were verified. In the PPI bar chart, the top 3 genes were chosen as hub genes involved in most pathways. On the TIMER and THPA websites, it is verified that the expression levels of CYBB, CD86, and C3AR1 genes in tumor tissues were higher than those in normal tissues. These genes may work as biomarkers or targets for accurate diagnosis and treatment of GC in the future. Our findings may be a new strategy for the treatment of GC.

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