Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase

Affiliations

Allison Judge: Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Liya Hu: Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Banumathi Sankaran: Department of Molecular Biophysics and Integrated Bioimaging, Berkeley Center for Structural Biology, Lawrence Berkeley National Laboratory
Justin Van Riper: Graduate Program in Chemical, Physical, and Structural Biology, Baylor College of Medicine
B. V. Venkataram Prasad: Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Timothy Palzkill: Department of Biochemistry and Molecular Biology, Baylor College of Medicine

Abstract

Structural analysis, antibiotic selection, and deep sequencing of CTX-M-14 mutants identify residues required for β-lactamase activity.