Parkinson’s disease alters the composition of subgingival microbiome

Ekin Yay; Melis Yilmaz; Hilal Toygar; Nur Balci; Carla Alvarez Rivas; Basak Bolluk Kilic; Ali Zirh; Bruce Paster; Alpdogan Kantarci

doi:10.1080/20002297.2023.2250650

Journal of Oral Microbiology (Dec 2023)

Parkinson’s disease alters the composition of subgingival microbiome

Ekin Yay,
Melis Yilmaz,
Hilal Toygar,
Nur Balci,
Carla Alvarez Rivas,
Basak Bolluk Kilic,
Ali Zirh,
Bruce Paster,
Alpdogan Kantarci

Affiliations

Ekin Yay: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA
Melis Yilmaz: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA
Hilal Toygar: Faculty of Dentistry, Department of Periodontology, Istanbul Medipol University, Istanbul, Turkey
Nur Balci: Faculty of Dentistry, Department of Periodontology, Istanbul Medipol University, Istanbul, Turkey
Carla Alvarez Rivas: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA
Basak Bolluk Kilic: Faculty of Dentistry, Department of Periodontology, Istanbul Medipol University, Istanbul, Turkey
Ali Zirh: Faculty of Medicine, Department of Neurology, Istanbul Medipol University, Istanbul, Turkey
Bruce Paster: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA
Alpdogan Kantarci: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA

DOI: https://doi.org/10.1080/20002297.2023.2250650
Journal volume & issue: Vol. 15, no. 1

Abstract

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ABSTRACTAim The current study aimed to test the hypothesis that Parkinson’s disease exacerbates periodontitis by altering its microbiome.Materials and Methods Clinical periodontal parameters were recorded. Subgingival samples from healthy controls, periodontitis patients (PD), and Parkinson’s patients with periodontitis (PA+PD) were analyzed using the checkerboard DNA-DNA hybridization technique for targeting 40 bacterial species typically associated with periodontal disease and health. Next-generation sequencing (NGS) of the 16S ribosomal RNA gene (V1-V3 regions) was performed to analyze the microbiome comprehensively.Results Parkinson’s patients had mild-to-moderate motor dysfunctions. Bleeding on probing was significantly increased in the PA+PD group compared to PD (p < 0.05). With checkerboard analysis, PA was associated with increased Treponema socranskii (p = 0.0062), Peptostreptococcaceae_[G-6] [Eubacterium]_nodatum (p = 0.0439), Parvimona micra (p < 0.0001), Prevotella melaninogenica (p = 0.0002), Lachnoanaerobaculum saburreum (p < 0.0001), and Streptococcus anginosus (p = 0.0020). Streptococcus intermedia (p = 0.0042), P.nodatum (p = 0.0022), P. micra (p = 0.0002), Treponema denticola (p = 0.0045), L.saburreum (p = 0.0267), P.melaninogenica (p = 0.0017), Campylobacter rectus (p = 0.0020), and T.socranskii (p = 0.0002) were higher; Aggregatibacter actinomycetemcomitans (p = 0.0072) was lower in deep pockets in the PA+PD compared to PD. Schaalia odontolytica (p = 0.0351) and A.actinomycetemcomitans (p = 0.002) were lower; C.rectus (p = 0.0002), P. micra (p = 0065), Streptococcus constellatus (p = 0.0151), T.denticola (p = 0.0141), P.melaninogenica (p = 0.0057), and T.socranskii (p = 0.0316) were higher in shallow pockets in the PA+PD. Diversity decreased in PD (p = 0.001) and PA+PD (p = 0.026) compared to control, with minimal differences in alpha and beta diversities among PD and PA+PD based on NGS results.Conclusion These data demonstrated that Parkinson’s disease modifies PD-associated subgingival microbiome.

Published in Journal of Oral Microbiology

ISSN: 2000-2297 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases; Science: Microbiology
Website: https://www.tandfonline.com/journals/zjom

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