Biological synthesis and characterization of Passiflora subpeltata Ortega aqueous leaf extract in silver nanoparticles and their evaluation of antibacterial, antioxidant, anti-cancer and larvicidal activities

Abstract

Passiflora subpeltata contain rich source of secondary metabolites and its plant materials mostly used for cancer and mosquito related problem. Therefore, in this study, green synthesis of silver nanoparticles using Passiflora subpeltata of aqueous extract (Ps-AgNPs) as reducing and stabilizing agent. The UV–Visible spectra of Ps-AgNPs showed absorption peak at 456 nm and formation of AgNPs. XRD pattern showed the crystalline nature and SEM images displayed predominantly spherical shape of NPs. FT-IR analysis exhibition capping of AgNPs by bioactive compounds from Ps-AgNPs. Antibacterial activity in PS-AgNPs shown good activity with best of B. cereus (27 mm) at higher dose of 75 μg/mL. Antioxidant activity was tested against DPPH, ABTS and H2O2 assays and the best scavenging assay were observed in DPPH with IC50 value of 108.92 μg/mL. The anti-proliferative activity in human colon cancer cell-line (HT-29) indicates that the significant result with IC50 value of 33.05 μg/mL. Larvicidal activity of PS-AgNPs showed good activity against Cx. quinquefasicatus larvae (LC50-4.99 units and LC90-22.19units). The green synthesized NPs could be potential activity and it used in different biomedical application.

Keywords

• Silver Nanoparticles
• FE-SEM
• H2O2
• Colon cancer
• Cx. quinquefasciatus