Probing the glioma micro-environment: Analysis using biopsy in combination with ultra-fast cyclic immunolabeling

Thomas S. van Solinge; Juhyun Oh; Erik Abels; Peter Koch; Xandra O. Breakefield; Ralph Weissleder; Marike L.D. Broekman

Neoplasia: An International Journal for Oncology Research (Nov 2024)

Probing the glioma micro-environment: Analysis using biopsy in combination with ultra-fast cyclic immunolabeling

Thomas S. van Solinge,
Juhyun Oh,
Erik Abels,
Peter Koch,
Xandra O. Breakefield,
Ralph Weissleder,
Marike L.D. Broekman

Affiliations

Thomas S. van Solinge: Departments of Neurology and Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA, USA; Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands
Juhyun Oh: Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA; Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Erik Abels: Departments of Neurology and Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA, USA; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands
Peter Koch: Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA
Xandra O. Breakefield: Departments of Neurology and Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA, USA
Ralph Weissleder: Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA; Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; Department of Systems Biology, Harvard Medical School, Boston, MA, USA; Corresponding author at: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.Corresponding author at: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.
Marike L.D. Broekman: Departments of Neurology and Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA, USA; Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands; Department of Neurosurgery, Haaglanden Medical Center, The Hague, the Netherlands; Corresponding author at: Department of Neurosurgery, Leiden University Medical Center, Albinusdreef 2 2333 ZA, Leiden, the Netherlands.

Journal volume & issue: Vol. 57
p. 101051

Abstract

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The interaction between gliomas and the immune system is poorly understood and thus hindering development of effective immunotherapies for glioma patients. The immune response is highly variable during tumor development, and affected by therapies such as surgery, radiation, and chemotherapy. Currently, analysis of these local changes is difficult due to poor accessibility of the tumor and high-morbidity of sampling. In this study, we developed a model for repeat-biopsy in mice to study these local immunological changes over time. Using fine needle biopsy we were able to safely and repeatedly collect cells from intracranial tumors in mice. Ultra-fast cycling technology (FAST) was used for multi-cycle immunofluorescence of retrieved cells, and provided insights in the changing immune response over time. The combination of these techniques can be utilized to study changes in the immune response in glioma or other intracranial diseases over time, and in response to treatment within the same animal.

Published in Neoplasia: An International Journal for Oncology Research

ISSN: 1522-8002 (Print); 1476-5586 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.journals.elsevier.com/neoplasia/

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Abstract

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