Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut
Kunkun Zhao,
Xufa Du,
Jingjing Zhang,
Yi Fan,
Qian Ma,
Yaoyao Li,
Zenghui Cao,
Di Cao,
Sasa Hu,
Ding Qiu,
Kai Zhao,
Fangping Gong,
Zhongfeng Li,
Rui Ren,
Lin Zhang,
Xingli Ma,
Xingguo Zhang,
Dongmei Yin
Affiliations
Kunkun Zhao
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Xufa Du
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Jingjing Zhang
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Yi Fan
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Qian Ma
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Yaoyao Li
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Zenghui Cao
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Di Cao
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Sasa Hu
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Ding Qiu
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Kai Zhao
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Fangping Gong
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Zhongfeng Li
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Rui Ren
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Lin Zhang
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Xingli Ma
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Xingguo Zhang
College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Dongmei Yin
Corresponding author.; College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450000, Henan, China
Pod size is a key agronomic trait that influences peanut yield greatly. However, our understanding of the mechanisms underlying pod size is limited. In this study, we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci (QTL) associated with pod size. Initial mapping performed using bulk segregant analysis revealed a candidate interval on chromosome A05 referred to as qPSW05. We refined this interval to a 256.9 kb genomic region using newly developed molecular markers. Through sequence and expression analyses, we identified the candidate gene AhXE45GC, which encodes an AN1 zinc finger protein. We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S. Accessions that lack this insertion, such as ND_L, had significantly larger pods than those with the insertion, including ND_S. To facilitate marker-assisted selection for peanut pod size, we developed a molecular marker associated with this polymorphism. This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.
