Adipose‐derived mesenchymal stem cell‐incorporated PLLA porous microspheres for cartilage regeneration

Abstract

Abstract Background In facial plastic surgery, patients with nasal deformity are often treated by rib cartilage transplantation. In recent years, cartilage tissue engineering has developed as an alternative to complex surgery for patients with minor nasal defects via injection of nasal filler material. In this study, we prepared an injectable nasal filler material containing poly‐L‐lactic acid (PLLA) porous microspheres (PMs), hyaluronic acid (HA) and adipose‐derived mesenchymal stem cells (ADMSCs). Methods We seeded ADMSCs into as‐prepared PLLA PMs using our newly invented centrifugation perfusion technique. Then, HA was mixed with ADMSC‐incorporated PLLA PMs to form a hydrophilic and injectable cell delivery system (ADMSC‐incorporated PMH). Results We evaluated the biocompatibility of PMH in vitro and in vivo. PMH has good injectability and provides a favorable environment for the proliferation and chondrogenic differentiation of ADMSCs. In vivo experiments, we observed that PMH has good biocompatibility and cartilage regeneration ability. Conclusion In this study, a injectable cell delivery system was successfully constructed. We believe that PMH has potential application in cartilage tissue engineering, especially in nasal cartilage regeneration.

Keywords

• centrifugation perfusion
• chondrogenesis
• hyaluronic acid
• nasal fillers
• PLLA porous microspheres