The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

Abstract

Jiamin Wen,1 Jitian Weng,2 Wei Lu,3 Xiaohua Tao,3 Hao Cheng,4,* Yi Tang3,* 1Department of Dermatology, People’s Hospital of Tiantai County, Zhejiang University, Taizhou, People’s Republic of China; 2School of Public Health, Hangzhou Normal University, Zhejiang University, Hangzhou, People’s Republic of China; 3Center for Plastic & Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Zhejiang University Hangzhou 310014 People’s Republic of China; 4Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yi Tang, Center for Plastic & Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Zhejiang University, 158 Shangtang Road, Xiacheng District, Hangzhou, 310014, People’s Republic of China, Tel [phone], Fax [phone], Email [email protected] Hao Cheng, Zhejiang University School of Medicine Sir Run Run Shaw Hospital, Hangzhou, Zhejiang, 310016, People’s Republic of China, Tel [phone], Fax [phone], Email [email protected]: To investigate the expression of Plasmacytoid Dendritic Cells (pDCs) and TLR7/9-MyD88-IRAKs pathway in chronic eczema lesions.Patients and Methods: Lesional tissues and the surrounding healthy tissues were collected from 25 individuals with chronic eczema, and immunohistochemistry was used to detect and comparatively analyze the expression profile of CD123, CD2AP, toll-like receptor 7 (TLR7), toll-like receptor 9 (TLR9) along with interleukin-1 receptor-associated kinase 1 (IRAK1) and interferon regulatory factor 7 (IRF7) in signaling pathways.Results: The positive rates of CD2AP + pDC and CD123 + pDC in lesional tissues were significantly elevated compared to the surrounding healthy tissues (P 0.05).Conclusion: PDC and TLR7/9-MyD88-IRAKs pathways are actively expressed in chronic eczema lesions and may be involved in pathogenesis and disease progression.Keywords: eczema, plasmacytoid dendritic cells, toll-like receptor 7, toll-like receptor 9, immunohistochemistry

Keywords

• eczema
• plasmacytoid dendritic cells
• toll-like receptor 7
• toll-like receptor 9
• immunohistochemistry