Elevated expression of NEDD9 is associated with metastatic activity in gastric cancer

Abstract

Si-sen Zhang,1 Li-hua Wu,1 Qing Liu,1 Kui-sheng Chen,2 Xie-fu Zhang31The Emergency Department, People’s Hospital of Zhengzhou, Zhengzhou, Henan, People’s Republic of China; 2Department of Pathology, 3General Surgery Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People’s Republic of ChinaObjective: To investigate the protein and mRNA expression of NEDD9 in gastric cancer (GC) tissues, adjacent atypical hyperplasia tissues, and normal gastric mucosa tissues, and analyze its relationship with the pathological features and prognosis of GC. Methods: Forty cases of GC tissues, 20 cases of adjacent atypical hyperplasia tissues, and 40 cases of normal gastric mucous tissues were collected. Immunohistochemistry and Western blot were used to examine the expression of NEDD9 protein in various tissues. Situ hybridization and reverse transcription polymerase chain reaction were applied to detect the expression of NEDD9 mRNA in various tissues. The correlation of NEDD9 expression with invasion and metastasis of GC was analyzed. Results: The protein expression level of NEDD9 was significantly higher in GC tissues than in adjacent atypical hyperplasia tissues and normal gastric mucous tissues (P<0.05). The protein expression level of NEDD9 was positively related to the invasion depth of carcinoma and tumor lymph node metastasis (P<0.05), but unrelated to age, sex, tumor size, and clinical classification of cancer (P>0.05). The mRNA expression level of NEDD9 was also significantly higher in GC tissues than in adjacent atypical hyperplasia tissues and normal gastric mucous tissues (P<0.05), and positively related with the tumor lymph node metastasis and invasion depth of carcinoma (P<0.05). Conclusion: NEDD9 is involved in the occurrence and development of GC, and it may be an important biological marker of GC metastasis and infiltration.Keywords: immunohistochemistry (IHC), invasion, metastasis