The Complete Genome Sequence of Pseudomonas syringae pv. actinidifoliorum ICMP 18803

Abstract

The genome of Pseudomonas syringae pv. actinidifoliorum ICMP18803 (Pfm) was sequenced using the Oxford Nanopore MinION platform to an average read depth of 123-fold. The genome assembled into a single chromosome of 6,353,853 bp after error correction with Illumina short reads using Pilon. No plasmids were detected. Despite Pfm only being a relatively weak pathogen of kiwifruit, a set of 31 effectors, 26 of which were full length, was identified by mapping the comprehensive effector library generated by previous researchers to the assembled chromosome. A revised Pfm genome with the effector complement correctly named and annotated was resubmitted to GenBank (CP081457). [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Keywords

• effector complement
• long-read assembly
• secondary metabolites