Applied Sciences (Jan 2021)

Antibacterial Behavior of Chitosan-Sodium Hyaluronate-PEGDE Crosslinked Films

  • Martha Gabriela Chuc-Gamboa,
  • Carolina María Cámara Perera,
  • Fernando Javier Aguilar Ayala,
  • Rossana Faride Vargas-Coronado,
  • Juan Valerio Cauich-Rodríguez,
  • Diana María Escobar-García,
  • Luis Octavio Sánchez-Vargas,
  • Neith Pacheco,
  • Julio San Román del Barrio

DOI
https://doi.org/10.3390/app11031267
Journal volume & issue
Vol. 11, no. 3
p. 1267

Abstract

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Chitosan is a natural polymer that can sustain not only osteoblast adhesion and proliferation for bone regeneration purposes, but it is also claimed to exhibit antibacterial properties towards several Gram-positive and Gram-negative bacteria. In this study, chitosan was modified with sodium hyaluronate, crosslinked with polyethylene glycol diglycidyl ether (PEGDE) and both osteoblast cytotoxicity and antibacterial behavior studied. The presence of sodium hyaluronate and PEGDE on chitosan was detected by FTIR, XRD, and XPS. Chitosan (CHT) films with sodium hyaluronate crosslinked with PEGDE showed a better thermal stability than pristine hyaluronate. In addition, osteoblast cytocompatibility improved in films containing sodium hyaluronate. However, none of the films exhibit antimicrobial activity against Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus while exhibiting low to mild activity against Salmonella typhimurion.

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