Resveratrol Supplementation Attenuates Excessive Inflammation and Helps Restore Impaired Restitution in an Intestinal Epithelial Cell Culture Model

Abstract

Aim:Sustained release of inflammatory mediators, excessive inflammatory response and impaired intestinal epithelial restitution are well-known mechanisms in the pathogenesis of necrotizing enterocolitis. This study focused on the effect of resveratrol on these pathways.Materials and Methods:In this study, the rat crypt intestinal cell line (IEC-6) culture, an application of lipopolysaccharide or a cytokine mixture and a scratch migration assay model were used. Nitric oxide synthase-2 (iNOS) and cyclooxygenase-2 (COX-2), focal adhesion kinase (FAK) and its phosphorylated form (pFAK) levels were assessed.Results:IEC-6 cells covered 88% of the denuded area in the control, 54% in LPS, and 35% in cytomix groups at the 24th hour. The treatment with resveratrol at doses of 0.5, 1 and 5 μM/L before LPS resulted in the repair of 84%, 87% and 76% of the denuded areas, respectively. Likewise, with cytomix, it was 86%, 82%, and 78%. Resveratrol at a dose of 5 μM/L prevented an increase in iNOS levels. All three doses of resveratrol were effective in preventing increases in COX levels. FAK or pFAK expressions remained unchanged in all groups.Conclusion:Resveratrol, being known for its antioxidant features, suppresses excessive inflammatory response and helps preservation of mucosal integrity by conservation of epithelial restitution.

Keywords

• necrotizing enterocolitis
• resveratrol
• restitution
• inflammation
• nitric oxide synthase-2