Secretion of 2,4 di-tertbutylphenol by a new Pseudomonas strain SBMCH11: A tert-butyl substituted phenolic compound displayed antibacterial efficacy

Abstract

Pseudomonas sp. SBMCH11 was newly isolated from maritime soil samples which was identified by morphological, biochemical, and 16S rRNA gene sequencing. The isolated Pseudomonas sp. SBMCH11 had significant antibacterial activity against clinical bacterial strains including Pseudomonas aeruginosa and Staphylococcus aureus. Subsequently, high concentrations of antibacterial substances were achieved by optimizing the following parameters: pH (7.0), carbon (1 %), nitrogen sources (casein 0.2 %), NaCl (10 %), and incubation time (120 hrs). Furthermore, the extracted component from the Pseudomonas sp. SBMCH11 was shown to have a significant inhibitory effect on tested clinical pathogens. On the other hand, the extracted compound was confirmed as 2,4 di-tertbutylphenol (2,4 DTBP) by GC–MS with a molecular weight of 206.5 Daltons. Based on these findings, the isolated 2,4 DTBP could be recognized as a promising bioactive molecule for further antibacterial medication development. Compounds identified in the maritime environment from microorganisms, especially Pseudomonas sp. SBMCH11 could be regarded as a potential bio-factory for the synthesis of antibacterial compounds to suppress large numbers of pathogenic bacteria in the natural environment.

Keywords

• Marine bacteria
• Antibacterial activity
• Clinical pathogens
• Pseudomonas species
• 2, 4 di-tertbutylphenol