Detection of Cauliflower mosaic virus by Serological and Molecular Methods in Northern and Razavi Khorasan Provinces of Iran

Abstract

Cauliflower mosaic virus is a type member of Caulimovirus from Caulimoviridae family that has double stranded DNA genome and is one of the most destructive viruses of cruciferous plants. In order to detect cauliflower mosaic virus in some areas of Iran under the cultivation of cauliflower, 343 symptomatic leaf samples of cauliflower were collected from Northern and Razavi Khorasan provinces of Iran in summer 2009-2010.To detect CaMV infection, collected plants were tested by DAS-ELISA. Polymerase chain reaction (PCR) using CaMV-specific primers (CMcp-F1\CMcp-R1) for ORF IV genes of CaMV was used for confirmation of infection. A 1400 bp fragment were amplified by PCR and then sequenced. Iranian isolates were compared with other isolates of CaMV deposited in the gene bank. The phylogenic trees of the coat protein genes of CaMV were drown by MEGA5 software using neighbor joining method. The results showed that the Iranian isolates clustered with Chinese and Hungarian isolates in a different clade from North- American isolates.

Keywords

• Cauliflower mosaic virus
• Coat protein
• DAS-ELISA
• PCR