MONITORING OF WATERMELON MOSAIC VIRUS 2 IN AGRIECOSYSTEMS OF KYIV AND POLTAVA REGIONS

Abstract

Aim. Monitoring of Watermelon mosaic virus 2 in agriecosystems of Kyiv and Poltava regions. Methods. Plant samples were analyzed using DAS- and indirect ELISA for presence of viral antigens. ELISA samples were prepared by standard methods. For Watermelon mosaic virus 2 (WMV-2) diagnostics, commercial antisera of INRA (France), Agdia (England) and Loewe (Germany) were used. Results. Following 9-year monitoring of Ukrainian agriecosystems we have demonstrated periodically changeable circulation of Watermelon mosaic virus 2 on plants of Cucurbitaceae family. It was determined that, in Ukraine, WMV 2-infected plants were typical only for open ground conditions. Watermelon mosaic virus 2 circulates mainly in form of mixed infection with Zucchini yellow mosaic virus, Cucumber mosaic virus or Cucurbit aphid-born yellows virus. Conclusions. In 2005, after nearly 30 years of absence, WMV-2 has been again detected on plants of Cucurbitaceae family in open-ground agriecosystems of Kyiv and Poltava regions of Ukraine. During the 9-year monitoring, WMV-2 has not been found at all on watermelon plants, though previously this pathogen was widely spread on watermelon plantings. In addition, it should be noticed we have never detected WMV-2 in glasshouse crops. Therefore in Ukraine WMV-2 remains a pathogen infecting exclusively the plants cultivated under open-ground conditions.

Keywords

• вірус мозаїки кавуна 2
• агроценози
• моніторинг
• змішана вірусна інфекція