Development of Submicrocapsules Based on Co-Assembled Like-Charged Silica Nanoparticles and Detonation Nanodiamonds and Polyelectrolyte Layers
Konstantin V. Palamarchuk,
Tatiana N. Borodina,
Anastasia V. Kostenko,
Yury M. Chesnokov,
Roman A. Kamyshinsky,
Natalya P. Palamarchuk,
Elena B. Yudina,
Elena D. Nikolskaya,
Nikita G. Yabbarov,
Mariia R. Mollaeva,
Tatiana V. Bukreeva
Affiliations
Konstantin V. Palamarchuk
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Tatiana N. Borodina
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, 59 Leninsky Pr., 119333 Moscow, Russia
Anastasia V. Kostenko
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Yury M. Chesnokov
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Roman A. Kamyshinsky
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Natalya P. Palamarchuk
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Elena B. Yudina
Ioffe Institute, 26 Politekhnicheskaya Str., 194021 St. Petersburg, Russia
Elena D. Nikolskaya
N.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia
Nikita G. Yabbarov
N.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia
Mariia R. Mollaeva
N.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia
Tatiana V. Bukreeva
National Research Centre “Kurchatov Institute”, 1 Akademika Kurchatova Sq., 123182 Moscow, Russia
Capsules with shells based on nanoparticles of different nature co-assembled at the interface of liquid phases of emulsion are promising carriers of lipophilic drugs. To obtain such capsules, theoretically using the Derjaguin–Landau–Verwey–Overbeek (DLVO) theory and experimentally using dynamic light-scattering (DLS) and transmission electron microscopy (TEM) methods, the interaction of like-charged silica nanoparticles and detonation nanodiamonds in an aqueous solution was studied and their ratios selected for the formation of submicron-sized colloidosomes. The resulting colloidosomes were modified with additional layers of nanoparticles and polyelectrolytes, applying LbL technology. As a model anti-cancer drug, thymoquinone was loaded into the developed capsules, demonstrating a significant delay of the release as a result of colloidosome surface modification. Fluorescence flow cytometry and confocal laser scanning microscopy showed efficient internalization of the capsules by MCF7 cancer cells. The obtained results demonstrated a high potential for nanomedicine application in the field of the drug-delivery system development.
