Frontiers in Pharmacology (Nov 2020)
Bruceine D inhibits Cell Proliferation Through Downregulating LINC01667/MicroRNA-138-5p/Cyclin E1 Axis in Gastric Cancer
- Lin Li,
- Lin Li,
- Lin Li,
- Lin Li,
- Lin Li,
- Zhen Dong,
- Zhen Dong,
- Zhen Dong,
- Zhen Dong,
- Zhen Dong,
- Pengfei Shi,
- Pengfei Shi,
- Pengfei Shi,
- Pengfei Shi,
- Li Tan,
- Li Tan,
- Li Tan,
- Li Tan,
- Jie Xu,
- Jie Xu,
- Jie Xu,
- Jie Xu,
- Pan Huang,
- Pan Huang,
- Pan Huang,
- Pan Huang,
- Zhongze Wang,
- Zhongze Wang,
- Zhongze Wang,
- Zhongze Wang,
- Hongjuan Cui,
- Hongjuan Cui,
- Hongjuan Cui,
- Hongjuan Cui,
- Hongjuan Cui,
- Liqun Yang,
- Liqun Yang,
- Liqun Yang,
- Liqun Yang
Affiliations
- Lin Li
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Lin Li
- Department of Immunology, School of Basic Medicine, Southwest Medical University, Luzhou, China
- Lin Li
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Lin Li
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Lin Li
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Zhen Dong
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Zhen Dong
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Zhen Dong
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Zhen Dong
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Zhen Dong
- NHC Key Laboratory of Birth Defects and Reproductive Health (Chongqing Key Laboratory of Birth Defects and Reproductive Health, Chongqing Population and Family Planning Science and Technology Research Institute), Chongqing, China
- Pengfei Shi
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Pengfei Shi
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Pengfei Shi
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Pengfei Shi
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Li Tan
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Li Tan
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Li Tan
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Li Tan
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Jie Xu
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Jie Xu
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Jie Xu
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Jie Xu
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Pan Huang
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Pan Huang
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Pan Huang
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Pan Huang
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Zhongze Wang
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Zhongze Wang
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Zhongze Wang
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Zhongze Wang
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Hongjuan Cui
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Hongjuan Cui
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Hongjuan Cui
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Hongjuan Cui
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- Hongjuan Cui
- NHC Key Laboratory of Birth Defects and Reproductive Health (Chongqing Key Laboratory of Birth Defects and Reproductive Health, Chongqing Population and Family Planning Science and Technology Research Institute), Chongqing, China
- Liqun Yang
- State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China
- Liqun Yang
- Cancer Center, Reproductive Medicine Center, Medical Research Institute, Southwest University, Chongqing, China
- Liqun Yang
- Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China
- Liqun Yang
- Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Chongqing, China
- DOI
- https://doi.org/10.3389/fphar.2020.584960
- Journal volume & issue
-
Vol. 11
Abstract
Objective: Gastric cancer is one of the most common malignant tumors. Bruceine D (BD) is one of the extracts of Brucea javanica. In recent years, it has been reported that BD has anti-tumor activity in some human cancers through different mechanisms. Here, this study try to explore the effect of BD on gastric cancer and its regulatory mechanism.Methods: Cell proliferation ability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, 5-bromo-2-deoxyuridine (BrdU) staining and soft agar colony formation assay, respectively. The tumor xenograft model was used to verify the effect of BD on the tumorigenicity of gastric cancer cells in vivo. Flow cytometry analysis and Western blot assay were performed to detect cell cycle and apoptosis. Gastric cancer cells were analyzed by transcriptome sequencing. The interaction between LINC01667, microRNA-138-5p (miR-138-5p) and Cyclin E1 was verified by dual luciferase experiment and RT-PCR assays.Results: We found that BD significantly inhibited cell proliferation and induced cell cycle arrest at S phase in gastric cancer cells. Transcriptome analysis found that the expression of a long non-coding RNA, LINC01667, were significantly down-regulated after BD treatment. Mechanically, it was discovered that LINC01667 upregulated the expression of Cyclin E1 by sponging miR-138-5p. Furthermore, BD enhanced the chemosensitivity of gastric cancer cells to doxorubicin, a clinically used anti-cancer agent.Conclusion: BD inhibit the growth of gastric cancer cells by downregulating the LINC01667/miR-138-5p/Cyclin E1 axis. In addition, BD enhances the chemosensitivity of gastric cancer cells to doxorubicin. This study indicates that BD may be used as a candidate drug for the treatment of patients with gastric cancer.
Keywords
