Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Abstract

Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease. The objective of this study was to evaluate the presence of nephrin mRNA (NPHS1) and podocin mRNA (NPHS2) in urine sediments of dogs with naturally occurring CKD and healthy dogs.

Keywords

• dog
• nphs1
• nphs2
• podocyturia
• quantitative real-time pcr