Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Jennifer Ngo; Ilan Y. Benador; Alexandra J. Brownstein; Laurent Vergnes; Michaela Veliova; Michael Shum; Rebeca Acín-Pérez; Karen Reue; Orian S. Shirihai; Marc Liesa

STAR Protocols (Mar 2021)

Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Jennifer Ngo,
Ilan Y. Benador,
Alexandra J. Brownstein,
Laurent Vergnes,
Michaela Veliova,
Michael Shum,
Rebeca Acín-Pérez,
Karen Reue,
Orian S. Shirihai,
Marc Liesa

Affiliations

Jennifer Ngo: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Department of Chemistry and Biochemistry at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Corresponding author
Ilan Y. Benador: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Corresponding author
Alexandra J. Brownstein: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Laurent Vergnes: Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Department of Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, USA
Michaela Veliova: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Michael Shum: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Rebeca Acín-Pérez: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Karen Reue: Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Department of Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, USA; Molecular Biology Institute at UCLA, Los Angeles, CA 90095, USA
Orian S. Shirihai: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Corresponding author
Marc Liesa: Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Molecular Biology Institute at UCLA, Los Angeles, CA 90095, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100243

Abstract

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Summary: Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry.For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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