OncoTargets and Therapy (2020-04-01)

The lncRNA LINC01194/miR-486-5p Axis Facilitates Malignancy in Non-Small Cell Lung Cancer via Regulating CDK4

  • Xing Z,
  • Zhang Z,
  • Gao Y,
  • Zhang X,
  • Kong X,
  • Zhang J,
  • Bai H

Journal volume & issue
Vol. Volume 13
pp. 3151 – 3163

Abstract

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Zhiwei Xing,1 Zhihua Zhang,2 Yanjun Gao,3 Xun Zhang,3 Xianglong Kong,3 Jianwu Zhang,3 Hongzhong Bai2 1Department of Blood Transfusion, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of China; 2Department of Radiology, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of China; 3Department of Laboratory, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaCorrespondence: Jianwu ZhangDepartment of Laboratory, Hebei Chest Hospital, No. 372 Shengli North Street, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaEmail [email protected] BaiDepartment of Radiology, Hebei Chest Hospital, No. 372 Shengli North Street, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaTel +86-0311 86911072Email [email protected]: This experimental design was based on lncRNA LINC01194 to explore the pathogenesis of NSCLC.Methods: RT-qPCR was used to detect the expression of lncRNA LINC01194 and miR-486-5p in NSCLC tissues and cell lines. CCK-8, colony formation, and transwell assays were used to examine the effects of lncRNA LINC01194 and miR-486-5p on NSCLC cell proliferation and migration invasiveness. For target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lncRNA LINC01194 and miR-486-5p. The protein expression of CDK4 was detected using Western blotting. The tumor changes in mice were detected by in vivo experiments in nude mice.Results: LncRNA LINC01194 was highly expressed in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975), and lncRNA LINC01194 significantly promoted cell proliferation and migration of NSCLC cells. MiR-486-5p was identified as a potential target for LINC01194, and miR-486-5p was expressed at a low level in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975). CDK4 was identified as a potential target for miR-486-5p. LncRNA LINC01194 was able to inhibit miR-486-5p expression and upregulate the expression level of CDK4. Finally, the results of in vivo animal models confirmed that lncRNA LINC01194 promoted NSCLC progression by modulating the miR-486-5p/CDK4 axis.Conclusion: LncRNA LINC01194 promoted the progression of NSCLC by modulating the miR-486-5p/CDK4 axis.Keywords: lncRNA LINC01194, miR-486-5p, CDK4, non-small cell lung cancer, proliferation, invasion

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