OncoTargets and Therapy (Apr 2020)
The lncRNA LINC01194/miR-486-5p Axis Facilitates Malignancy in Non-Small Cell Lung Cancer via Regulating CDK4
Abstract
Zhiwei Xing,1 Zhihua Zhang,2 Yanjun Gao,3 Xun Zhang,3 Xianglong Kong,3 Jianwu Zhang,3 Hongzhong Bai2 1Department of Blood Transfusion, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of China; 2Department of Radiology, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of China; 3Department of Laboratory, Hebei Chest Hospital, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaCorrespondence: Jianwu ZhangDepartment of Laboratory, Hebei Chest Hospital, No. 372 Shengli North Street, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaEmail [email protected] BaiDepartment of Radiology, Hebei Chest Hospital, No. 372 Shengli North Street, Shijiazhuang City, Hebei Province 050041, People’s Republic of ChinaTel +86-0311 86911072Email [email protected]: This experimental design was based on lncRNA LINC01194 to explore the pathogenesis of NSCLC.Methods: RT-qPCR was used to detect the expression of lncRNA LINC01194 and miR-486-5p in NSCLC tissues and cell lines. CCK-8, colony formation, and transwell assays were used to examine the effects of lncRNA LINC01194 and miR-486-5p on NSCLC cell proliferation and migration invasiveness. For target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lncRNA LINC01194 and miR-486-5p. The protein expression of CDK4 was detected using Western blotting. The tumor changes in mice were detected by in vivo experiments in nude mice.Results: LncRNA LINC01194 was highly expressed in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975), and lncRNA LINC01194 significantly promoted cell proliferation and migration of NSCLC cells. MiR-486-5p was identified as a potential target for LINC01194, and miR-486-5p was expressed at a low level in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975). CDK4 was identified as a potential target for miR-486-5p. LncRNA LINC01194 was able to inhibit miR-486-5p expression and upregulate the expression level of CDK4. Finally, the results of in vivo animal models confirmed that lncRNA LINC01194 promoted NSCLC progression by modulating the miR-486-5p/CDK4 axis.Conclusion: LncRNA LINC01194 promoted the progression of NSCLC by modulating the miR-486-5p/CDK4 axis.Keywords: lncRNA LINC01194, miR-486-5p, CDK4, non-small cell lung cancer, proliferation, invasion
