An SNP-Based Genetic Map and QTL Mapping for Growth Traits in the Red-Spotted Grouper (<i>Epinephelus akaara</i>)

Xiang Wang; Shoujia Jiang; Leyun Zheng; Ling Xiao; Xinhui Zhang; Dengdong Wang; Shuisheng Li; Qiong Shi; Shuiqing Wu; Haoran Lin; Xinxin You; Yong Zhang

doi:10.3390/genes10100793

Genes (Oct 2019)

An SNP-Based Genetic Map and QTL Mapping for Growth Traits in the Red-Spotted Grouper (<i>Epinephelus akaara</i>)

Xiang Wang,
Shoujia Jiang,
Leyun Zheng,
Ling Xiao,
Xinhui Zhang,
Dengdong Wang,
Shuisheng Li,
Qiong Shi,
Shuiqing Wu,
Haoran Lin,
Xinxin You,
Yong Zhang

Affiliations

Xiang Wang: South China Sea Marine Survey and Technology Center, SOA, Guangzhou 510275, China
Shoujia Jiang: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
Leyun Zheng: Fisheries Research Institute of Fujian, Xiamen 361012, China
Ling Xiao: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China
Xinhui Zhang: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
Dengdong Wang: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China
Shuisheng Li: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China
Qiong Shi: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
Shuiqing Wu: Fisheries Research Institute of Fujian, Xiamen 361012, China
Haoran Lin: Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, China
Xinxin You: Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
Yong Zhang: Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, China

DOI: https://doi.org/10.3390/genes10100793
Journal volume & issue: Vol. 10, no. 10
p. 793

Abstract

Read online

The red-spotted grouper (Epinephelus akaara) is one of the most commercially important aquatic species in China. However, its seedstock has low larval survival rates, and its stability is confronted with the danger of overexploitation. In this study, a high-density genetic map was constructed using 3435 single nucleotide polymorphisms (SNPs) from 142 first generation (F1) full-sib offspring and two parents of a red-spotted grouper population. The total genetic length of the map was 2300.12 cM with an average intermarker distance of 0.67 cM. Seventeen genome-wide significant quantitative trait loci (QTLs) for growth-related traits were detected on 24 linkage groups, including 5 QTLs for full length, 7 QTLs for body length, and 5 QTLs for body weight. The contribution values of explained phenotypic variance ranged from 10.7% to 12.9%. Moreover, 13 potential candidate genes for growth-related traits were identified. Collectively, these findings will be useful for conducting marker-assisted selection of the red-spotted grouper in future studies.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

About the journal

Abstract

Keywords