Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

Daniella C. Terenzi; Ehab Bakbak; Justin Z. Trac; Mohammad Al-Omran; Adrian Quan; Hwee Teoh; Subodh Verma; David A. Hess

STAR Protocols (Mar 2021)

Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

Daniella C. Terenzi,
Ehab Bakbak,
Justin Z. Trac,
Mohammad Al-Omran,
Adrian Quan,
Hwee Teoh,
Subodh Verma,
David A. Hess

Affiliations

Daniella C. Terenzi: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Corresponding author
Ehab Bakbak: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada
Justin Z. Trac: Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada
Mohammad Al-Omran: Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Department of Surgery, University of Toronto, Toronto, ON, Canada
Adrian Quan: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada
Hwee Teoh: Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Division of Endocrinology and Metabolism, Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada
Subodh Verma: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Department of Surgery, University of Toronto, Toronto, ON, Canada
David A. Hess: Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Molecular Medicine Research Laboratories, Robarts Research Institute, London, ON, Canada; Department of Physiology and Pharmacology, Western University, London, ON, Canada; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100311

Abstract

Read online

Summary: The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously.For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords