Medicina v Kuzbasse (Nov 2022)
THE STATE OF THE MICROCIRCULATORY BED IN DIABETIC FOOT SYNDROME WITH THE CARRIAGE OF VARIOUS VARIANTS OF THE T1565C POLYMORPHISM GENOTYPES OF THE ITGB3 GENE
Abstract
Purpose of the work – to study the state of the microvasculature in patients with the development of diabetic foot syndrome with various variants of carriage of the T1565C polymorphism genotypes of the ITGB3 gene. Materials and methods. The study involved 198 patients with uncomplicated diabetes mellitus and 199 patients with diabetic foot syndrome, in whom the distribution of genotypes of the T1565C polymorphic marker of the ITGB3 gene was studied by polymerase chain reaction. In 30 people in each group, comparable in distribution of polymorphisms of the studied gene, the state of the microvasculature was examined at three points using laser Doppler flowmetry. Results. A decrease in the average fluctuation of perfusion was revealed in the T/T and T/C genotypes of the T1565C polymorphism of the ITGB3 gene in diabetic foot at the point on the forearm compared with similar genotypes in diabetes mellitus. With the T/T genotype of T1565C polymorphism of the ITGB3 gene in diabetic foot, a decrease in the respiratory amplitude of microcirculation oscillations was noted. At the point on the back of the foot with the T/T genotype of the T1565C polymorphism of the ITGB3 gene, a decrease in the indicator characterizing microcirculation was noted. With the T/C genotype of T1565C polymorphism of the ITGB3 gene at a point on the 1st toe in diabetic foot, a decrease in the average value of the microcirculatory bed index and an increase in the coefficient of variation were revealed. Conclusion. The data obtained may indicate the presence of genetically determined changes in the state of microcirculation at various levels of the microcirculatory bed in diabetic foot syndrome. In addition, it can be assumed that the presence of single nucleotide substitutions in the ITGB3 gene is associated with different ways of implementing the mechanisms of microcirculatory disorders, which may be an essential component of the pathogenesis of this complication of diabetes mellitus.
