Food and Agricultural Immunology (Jan 2018)

Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples

  • Daniel Mukunzi,
  • Steven Suryoprabowo,
  • Shanshan Song,
  • Liqiang Liu,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1406460
Journal volume & issue
Vol. 29, no. 1
pp. 484 – 497

Abstract

Read online

An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food.

Keywords