Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki (Dec 2019)
Reactivity of the lymphoid component of the periodontium using various filing materials against experimental diabetes
Abstract
The purpose. The features of the restructuring of the lymphoid component of periodontal disease in experimental diabetes mellitus and in the combination with experimental diabetes mellitus with the presence of photopolymer or cement fillings were studied in this research. Materials and methods. The histological, immunohistochemical and lectohistochemical methods were used, the number of antigen-presenting cells, T and B lymphocytes in the periodontium during six weeks was studied. Results. In experimental diabetes mellitus, the number of SBA+-B-lymphocytes doubles than normal. The combination of photopolymer fillings and experimental diabetes mellitus led to an increase in the number of subpopulations of CD4+-, CD8+-lymphocytes by 15–20 %. At the same time, the number of intraepithelial HPA+-lymphocytes increased, which indicates the activation of the cellular immunity unit. In experimental diabetes mellitus, during the entire observation period, there was an increase in the number of subpopulations of CD4+-lymphocytes, compared with the control group. Their number was 4,01 ± 0,01 per conventional unit of area. Conclusions. In animals of the intact group, the periodoid lymphoid component is represented by antigen-presenting cells, CD5+-, CD4+-, CD8+-lymphocytes of the gum epithelium and lymphocytes of the lamina propria. In experimental diabetes mellitus, activation of antigen-presenting cells, cellular and humoral immunity is observed. When comparing the reactivity of the lymphoid component of the gums, the greatest shifts are observed in the presence of a photopolymer filling, in contrast to the cement, especially the humoral immunity. With a combination of experimental diabetes mellitus and the presence of filling material, more pronounced changes in the structure of the lymphoid component are noted in the presence of a photopolymer filling than cement.
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