Generation and characterization of two iPSC lines from human epicardium-derived cells

Christina Paulitschek; Peggy Schulze-Matz; Julia Hesse; Timo Schmidt; Wasco Wruck; James Adjaye; Jürgen Schrader

doi:10.1016/j.scr.2017.02.007

Stem Cell Research (Apr 2017)

Generation and characterization of two iPSC lines from human epicardium-derived cells

Christina Paulitschek,
Peggy Schulze-Matz,
Julia Hesse,
Timo Schmidt,
Wasco Wruck,
James Adjaye,
Jürgen Schrader

Affiliations

Christina Paulitschek: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
Peggy Schulze-Matz: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
Julia Hesse: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
Timo Schmidt: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
Wasco Wruck: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
James Adjaye: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany
Jürgen Schrader: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany

DOI: https://doi.org/10.1016/j.scr.2017.02.007
Journal volume & issue: Vol. 20, no. C
pp. 50 – 53

Abstract

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Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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