Genetic relationships between 4 Parkia spp. and variation in Parkia speciosa Hassk. based on random amplified polymorphic DNA (RAPD) markers

Abstract

A study was undertaken by RAPD to investigate genetic relationships among the following 4 Parkia species, Sator(Parkia speciosa Hassk.), Riang (P. timoriana Merr.), Khonkhong (P. leiophylla Kurz) and Lukding (P. sumatrana Miq.)and two plants of Parkia spp., namely Tong and Tien were also included. DNA from the leaf samples was isolated usingCTAB. Of total 180 primers first screened, 8 primers were chosen to analyse genetic variation of 103 individual plants. Atotal of 125 amplified fragments was obtained from 8 primers with an average of 15.63 fragments per primer, of which 101fragments (80.80%) were polymorphic. Some species-specific fragments were also obtained. With P. speciosa Hassk., 77polymorphic fragments (68.75%) were obtained with an average of 14 fragments per primer, but no specific fragment coulddifferentiate Sator khao from Sator dan. Results from the dendrogram revealed 5 groups of Parkia with similarity coefficientsranging from 0.437-1.000. Tien was found to be in the same group as Riang while Tong could be separated from otherParkia with a close relationship to Khonkhong at a similarity value 0.725.

Keywords

• Parkia spp.
• RAPD
• genetic relationship
• similarity coefficient