Molecular Detection of Chlamydophila Abortus In Aborted Fetal Tissues by Using Polymerase Chain Reaction (PCR) In Tabriz, Northwest of Iran

Abstract

Enzootic abortion of ewes (EAE) induced by Chlamydophila abortus (formerly Chlamydia psittaci serotype1) is a major cause of reproductive failures in most sheep producing countries. In this study, the abortion prevalence of Chlamydophila abortus (C. abortus) in sheep abortion cases are evaluated by polymerase chain reaction (PCR) and objectives are considered Chlamydiosis incidence of abortion in sheep in the northwest region of Iran. For this purpose, the contents of fetal tissue from 50 aborted fetuses were homogenized and DNA extracted from them and then PCR is done using specific primers for Chlamydophila (CHOMP191, CHOMP371). The study showed that about 26% of the total sample is contained Chlamydophila. This study confirms that C. abortus as an important cause of ovine abortion in the northwest of Iran and showed the PCR in tissue pools of aborted fetuses is a useful method for rapid detection of Chlamydophila abortus Ovis infections.

Keywords

• chlamydophila abortus
• molecular detection
• polymerase chain reaction (pcr)