Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus <i>Cymostachys</i> sp. NBUF082
Ye Yuan,
Te Li,
Tingting Wang,
C. Benjamin Naman,
Jing Ye,
Xingxin Wu,
J. Enrico H. Lazaro,
Xiaojun Yan,
Shan He
Affiliations
Ye Yuan
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
Te Li
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
Tingting Wang
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
C. Benjamin Naman
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
Jing Ye
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
Xingxin Wu
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
J. Enrico H. Lazaro
National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon 1101, Philippines
Xiaojun Yan
School of Marine Science, Ningbo University, Ningbo 315832, China
Shan He
Department of Marine Pharmacy, Li Dak Sum Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Island. The constitution and configuration of 1 was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey’s analysis and chiral HPLC. It was observed that 1 was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC50 value of 9.2 ± 1.1 μM.
