Effects of free-air CO2 enrichment on flower opening time in rice

Abstract

Flower opening time (FOT) is important for reproductive success in higher plants. Rice plants exhibit phenotypic plasticity in FOT in response to environmental factors such as temperature. However, the effect of the concentration of atmospheric CO2 ([CO2]) on FOT has not yet been reported. Elevated [CO2] (E-[CO2]) increases the temperature of panicles, which may in turn advance FOT. We investigated the effect of E-[CO2] on FOT in rice using a free-air CO2 enrichment facility where we increased [CO2] by about 200 μmol mol−1 above the ambient level (A-[CO2]). By photographing panicles at 10-min intervals, we determined 10%FOT and 50%FOT (the time of the day in Japan Standard Time when 10% and 50% of the flowers had opened, respectively). E-[CO2] advanced 10%FOT and 50%FOT by 4 and 5 min at the 10% and 5% levels of significance, respectively. Daily mean air temperature (Ta), solar radiation, and vapor-pressure deficit were negatively correlated with 50%FOT. Regression line slopes for Ta versus 10%FOT and 50%FOT were slightly steeper for A-[CO2] than those for E-[CO2]. Our results suggest that the most probable reason why E-[CO2] advanced FOT is an increase in panicle temperature arising from a reduction in leaf stomatal conductance. Abbreviations: 10%FOT: the time of the day in Japan Standard Time when 10% of the flowers had opened; 50%FOT: the time of the day in Japan Standard Time when 50% of the flowers had opened; A-[CO2]: ambient CO2; [CO2]: the concentration of atmospheric carbon dioxide; E-[CO2]: elevated [CO2]; FACE: free-air CO2 enrichment; FOT: flower opening time; JST: Japan Standard Time; RH: relative humidity; Rs: solar radiation; Ta: air temperature; Tp: panicle temperature; VPD: vapor-pressure deficit

Keywords

• air temperature
• elevated [co2]
• flower opening time
• free-air co2 enrichment
• heat stress
• rice