BMC Infectious Diseases (Dec 2024)

Epidemiological and genetic characteristics of clinical carbapenemase-producing Enterobacterales isolates from Batna hospitals in Algeria

  • Amel Benbouza,
  • Ahmed Kassah-Laouar,
  • Widad Chelaghma,
  • Fayza Bouziane,
  • Yassina Mebarki,
  • Jean-Marc Rolain,
  • Lotfi Loucif

DOI
https://doi.org/10.1186/s12879-024-10300-8
Journal volume & issue
Vol. 24, no. 1
pp. 1 – 11

Abstract

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Abstract Background Carbapenemase-producing Enterobacterales isolates are associated with significant mortality and have emerged as a major problem in healthcare settings worldwide. Objective Our aim was to investigate the epidemiological and genotypic characteristics of carbapenemase-positive Enterobacterales isolates from patients hospitalised in three hospitals in the city of Batna, Algeria. Methods Between 2016 and 2019, a total of 5,316 clinical isolates were obtained. The collected isolates were identified using the VITEK-2 system. Demographic and microbiological data were collected as well as the antimicrobial susceptibility testing, phenotypic and molecular characterisation of carbapenemase and mcr-1 genes were performed. Results Out of the 5,316 isolates, 201 were carbapenem-resistant Enterobacterales isolates and 179 of them (89.05%) were positive for the production of carbapenemase, of which Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae were the most common. The bla OXA−48−like gene alone was detected in 147 isolates (82.12%) moreover, the bla NDM gene was detected in ten isolates (5.59%). Dual and triple combinations of carbapenemase genes were also observed here for the first time in Algeria: bla VIM and bla OXA−48−like; bla KPC, bla VIM and bla OXA−48−like; bla VIM and bla NDM; bla KPC, bla NDM and bla VIM; bla NDM and bla OXA−48−like genes. In addition, resistance to both colistin and carbapenem antibiotics was detected in eight isolates, however none of them was positive for the mcr-1 gene. Conclusion This is the first study reporting the detection of carbapenemase genes in Klebsiella aerogenes, Enterobacter sakazakii, Raoultella ornithinolytica, Serratia ficaria, and Serratia marcescens and specific carbapenemase gene combinations in Algeria. The present study revealed that bla OXA−48−like were found to be the predominant carbapenemase genes in Batna hospitals.

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