BIO Web of Conferences (Jan 2023)

Real-time multiplex PCR method for detection of A. veronii A. caviae A. salmonicida

  • Feoktisova Natalia A.,
  • Nafeev Alexander A.,
  • Mastilenko Andrey V.,
  • Bogdanov Ilgizar I.,
  • Lomakin Artyom A.,
  • Minaeva Angelina N.,
  • Rodionova Arina V.

DOI
https://doi.org/10.1051/bioconf/20237101076
Journal volume & issue
Vol. 71
p. 01076

Abstract

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As a result of the experiments, the parameters for setting up a polymerase chain reaction were developed for the accelerated identification of Aeromonas spp. The design of species-specific primers was performed, the reaction parameters were selected, the specificity was checked, and the optimal amplification time was selected. As a result of this study, a multiplex real-time PCR test system was developed for the simultaneous detection of A. salmonicida, A. caviae and A. veronii. The sensitivity of the developed protocol was 5.56 pg/µl. The data obtained will allow for accelerated screening of these pathogens.