Real-time multiplex PCR method for detection of A. veronii A. caviae A. salmonicida

Abstract

As a result of the experiments, the parameters for setting up a polymerase chain reaction were developed for the accelerated identification of Aeromonas spp. The design of species-specific primers was performed, the reaction parameters were selected, the specificity was checked, and the optimal amplification time was selected. As a result of this study, a multiplex real-time PCR test system was developed for the simultaneous detection of A. salmonicida, A. caviae and A. veronii. The sensitivity of the developed protocol was 5.56 pg/µl. The data obtained will allow for accelerated screening of these pathogens.