Frontiers in Microbiology (Jan 2023)
Butyrate limits the replication of porcine epidemic diarrhea virus in intestine epithelial cells by enhancing GPR43-mediated IFN-III production
- Haiyan He,
- Haiyan He,
- Xuelei Fan,
- Xuelei Fan,
- Haiyan Shen,
- Haiyan Shen,
- Haiyan Shen,
- Hongchao Gou,
- Hongchao Gou,
- Hongchao Gou,
- Chunhong Zhang,
- Chunhong Zhang,
- Chunhong Zhang,
- Zhicheng Liu,
- Zhicheng Liu,
- Zhicheng Liu,
- Zhicheng Liu,
- Bin Zhang,
- Bin Zhang,
- Nile Wuri,
- Nile Wuri,
- Jianfeng Zhang,
- Jianfeng Zhang,
- Jianfeng Zhang,
- Ming Liao,
- Ming Liao,
- Ming Liao,
- Letu Geri
Affiliations
- Haiyan He
- College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China
- Haiyan He
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Xuelei Fan
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Xuelei Fan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Haiyan Shen
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Haiyan Shen
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Haiyan Shen
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Hongchao Gou
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Hongchao Gou
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Hongchao Gou
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Chunhong Zhang
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Chunhong Zhang
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Chunhong Zhang
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Zhicheng Liu
- College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China
- Zhicheng Liu
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Zhicheng Liu
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Zhicheng Liu
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Bin Zhang
- College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China
- Bin Zhang
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Nile Wuri
- College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China
- Nile Wuri
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Jianfeng Zhang
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Jianfeng Zhang
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Jianfeng Zhang
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Ming Liao
- Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Ming Liao
- Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, China
- Ming Liao
- Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Letu Geri
- College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China
- DOI
- https://doi.org/10.3389/fmicb.2023.1091807
- Journal volume & issue
-
Vol. 14
Abstract
Porcine epidemic diarrhea virus (PEDV) is a threat to the health of newborn piglets and has a significant impact on the swine industry. Short-chain fatty acids (SCFAs) are gut microbial metabolites that regulate intestinal function through different mechanisms to enhance the intestinal barrier and immune function. In this study, we aimed to determine whether butyrate displayed a better effect than other SCFAs on limiting PEDV replication in porcine intestinal epithelial cells. Mechanistically, butyrate treatment activated the interferon (IFN) response and interferon-stimulated gene (ISG) expression. Further experiments showed that inhibition of GPR43 (free fatty acid receptor 2) in intestinal epithelial cells increased virus infection and reduced antiviral effects through IFN λ response. Our findings revealed that butyrate exerts its antiviral effects by inducing GPR43-mediated IFN production in intestinal epithelial cells.
Keywords
