University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, USADepartment of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, USA
Gutian Xiao
University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, USADepartment of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, USA
Zhaoxia Qu
University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, USADepartment of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, USA
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells, pathogens, proteins such as cytokines/chemokines, and the expression levels of inflammation-related and other genes in the cells. This will help to understand the underlying mechanisms of these lung diseases and develop specific and effective drugs.
