Determination of Ethylene Glycol Dinitrate in Environmental and Forensic Water Samples Using Microextraction by Packed Sorbent Followed by Gas Chromatography

Abstract

Ethylene glycol dinitrate (EGDN) is a liquid nitrate ester, a secondary explosive. In the past, it was used as an explosive ingredient in dynamite along with nitroglycerine. Due to its various applications, the reliable detection of EGDN in the environment is a key issue for both forensic and environmental applications. In these areas, sensitive and reliable methods for determining the concentration of nitro compounds are needed. Microextraction by packed sorbent (MEPS) is an innovative approach to green technology in the sample preparation field. Compared to conventional solid-phase extraction (SPE), MEPS uses a smaller sample volume and can be easily combined with various chromatographic techniques. An important benefit is the reduction of sorbent amount and up to 100-times repeatable use compared to disposable SPE columns, thus reducing the costs of analysis as well as waste production. Optimal extraction parameters for isolating EGDN from water, e.g., 30 µL of toluene as extraction agent, working in one cycle and in draw/discard mode, were selected. Method validation was performed, obtaining a limit of detection and a limit of quantification of 0.45 pg/μL and 1.34 pg/μL, respectively. Accuracy in terms of recovery rates was evaluated over a wide concentration range, obtaining values from 83.7 to 90.0%. The satisfactory linearity expressed by the coefficient of determination was 0.9914. A matrix factor of −9.3% indicates a weak matrix effect. The application to real environmental water samples and a forensic post-blast wash water sample was realized. EGDN detection in the post-blast samples provides valuable information for forensic technicians.

Keywords

• environmental water
• ethylene glycol dinitrate
• forensic post-blast residues
• gas chromatography
• microextraction by packed sorbent