High speed counter current chromatography: A support-free LC technique

Abstract

As separation of components is the major requirement of an analytical chemist, there is always a need for a convenient high throughput technique with minimum sample loss, high efficiency, high resolution, and ease of sample recovery, without contamination. This leads to the development of High Speed Counter Current Chromatography (HSCCC), in which the stationary phase is liquid instead of solid, and that provides a lot of advantages over other chromatographic techniques. In addition, advanced centrifugal partition technology is used to hold the liquid stationary phase in the column, while the liquid mobile phase is pushed through it, which provides high yield and purity. This review highlights the major applications of HSCCC that include extraction of medicinal drugs from plants and purification and isolation of active material, plant analysis, separation of rare earth elements, preparative-scale separations of chiral compounds, analysis of inorganic compounds and elements, drug discovery, and drug development. Separation of dipeptides and proteins, flavonoids, alkaloids, DNP amino acids, indole auxins, and so on, proves the versatile and dynamic nature of the technique.

Keywords

• Centripetal accelerations
• high speed counter current chromatography
• solvent system