Bio-Protocol (Dec 2016)

Bacterial Growth Inhibition Assay for Xanthomonas oryzae pv. oryzae or Escherichia coli K12 Grown together with Plant Leaf Extracts

  • Marco Loehrer,
  • Rhoda Delventhal,
  • Denise Weidenbach,
  • Ulrich Schaffrath

DOI
https://doi.org/10.21769/BioProtoc.2069
Journal volume & issue
Vol. 6, no. 24

Abstract

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We performed a growth inhibition assay to test antibacterial compounds in leaf extracts from transgenic rice plants. The assay is based on over-night co-incubation of a defined concentration of colony forming units (cfu) of the respective bacteria together with aqueous extracts of ground leaf tissue.