Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Pavlova Maria R.; Dobreva Elina G.; Ivanova Katucha I.; Asseva Galina D.; Ivanov Ivan N.; Petrov Peter K.; Velev Valeri R.; Tomova Ivelina I.; Tiholova Maida M.; Kantardjiev Todor V.

doi:10.1515/folmed-2016-0016

Folia Medica (Jun 2016)

Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Pavlova Maria R.,
Dobreva Elina G.,
Ivanova Katucha I.,
Asseva Galina D.,
Ivanov Ivan N.,
Petrov Peter K.,
Velev Valeri R.,
Tomova Ivelina I.,
Tiholova Maida M.,
Kantardjiev Todor V.

Affiliations

Pavlova Maria R.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria, Tel.: +359 898 639 929
Dobreva Elina G.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria
Ivanova Katucha I.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria
Asseva Galina D.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria
Ivanov Ivan N.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria
Petrov Peter K.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria
Velev Valeri R.: Hospital for Infectious and Parasitic Diseases (HIPD), Medical University of Sofia, Bulgaria
Tomova Ivelina I.: Hospital for Infectious and Parasitic Diseases (HIPD), Medical University of Sofia, Bulgaria
Tiholova Maida M.: Hospital for Infectious and Parasitic Diseases (HIPD), Medical University of Sofia, Bulgaria
Kantardjiev Todor V.: National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, Bulgaria

DOI: https://doi.org/10.1515/folmed-2016-0016
Journal volume & issue: Vol. 58, no. 2
pp. 95 – 100

Abstract

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Introduction: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods.

Published in Folia Medica

ISSN: 0204-8043 (Print); 1314-2143 (Online)
Publisher: Pensoft Publishers
Country of publisher: Bulgaria
LCC subjects: Medicine
Website: https://foliamedica.bg

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