Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid

Abstract

Purpose: The aim of this study was to investigate the effects of Inula viscosa extract and Curcumin on the U87 (human astrocytoma cell line) treated with amyloid-beta (Aβ), which is the Alzheimer’s disease (AD) model cell line. Materials and Methods: Firstly, the cytotoxic potential of ınula and curcumin was investigated in the U87 cells by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay. Then, the amount of Total Glutathione, Malondialdehyde (MDA), Glutathione reductase (GR) activities were investigated. ELISA test was used to examine the expression and activity of cleaved Bax and Bcl-2 proteins in the Inula viscosa and Curcumin treated U87 cell lines. Results: Inula viscosa and Curcumin treatment reduced cell death caused by amyloid-B in cells. It also reduced the oxidative stress caused by amyloid-B, while reducing the activation of the proapoptotic protein Bax, and Bcl-2. Conclusion: Our results suggest that inula viscosa may represent a new approach in the treatment of Alzheimer's.

Keywords

• ınula viscosa
• curcumin
• alzheimer
• u87
• ınula viscosa
• curcumin
• u87
• alzheimer