Development of Nano–Micro Fused LSPR Chip for In Situ Single-Cell Secretion Analysis
Yuhei Terada,
Ain Obara,
Jonathan Campos Briones,
Xi Luo,
Wilfred Villariza Espulgar,
Masato Saito,
Hyota Takamatsu,
Eiichi Tamiya
Affiliations
Yuhei Terada
Environmental Management Research Institute (EMRI), Department of Energy and Environment, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8569, Ibaraki, Japan
Ain Obara
Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Suita 565-0871, Osaka, Japan
Jonathan Campos Briones
Life and Medical Photonics Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita 565-0871, Osaka, Japan
Xi Luo
Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Suita 565-0871, Osaka, Japan
Wilfred Villariza Espulgar
Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Suita 565-0871, Osaka, Japan
Masato Saito
Life and Medical Photonics Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita 565-0871, Osaka, Japan
Hyota Takamatsu
Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita 565-0871, Osaka, Japan
Eiichi Tamiya
Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Suita 565-0871, Osaka, Japan
Single-cell analysis has become increasingly important in uncovering cell heterogeneity, which has great implications in medicine and biology for a deep understanding of cell characteristics. Owing to its significance, it is vital to create novel devices that can reveal special or unique cells. In this work, we developed a single-cell secretion detection chip consisting of microwells that can trap single cells. Each well is surrounded by Au nanopillars capable of localized surface plasmon resonance (LSPR) measurement. Using microfabrication and nanofabrication techniques, Au nanopillar and microwell structures were fabricated on a COP film. The Au nanopillar was modified with IL-6 antibodies for the direct detection of single-cell secreted IL-6 via LSPR absorbance peak shift. Specific IL-6 detection was successfully demonstrated using a null and IL-6 oversecreting Jurkat cell. A high single-cell trapping efficiency of over 80% was also achieved. Overall, the development of this single-cell secretion detection chip with a simple LSPR measurement setup represents a significant development in the field of cell biology and immunology, providing researchers with a powerful tool for studying individual cells and their secreted cytokines, and is useful for point-of-care testing (POCT) diagnostics.
