Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern

Danique M. H. van Rijswijck; Albert Bondt; Max Hoek; Karlijn van der Straten; Tom G. Caniels; Meliawati Poniman; Dirk Eggink; Chantal Reusken; Godelieve J. de Bree; Rogier W. Sanders; Marit J. van Gils; Albert J. R. Heck

doi:10.1038/s41467-022-33899-1

Nature Communications (Oct 2022)

Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern

Danique M. H. van Rijswijck,
Albert Bondt,
Max Hoek,
Karlijn van der Straten,
Tom G. Caniels,
Meliawati Poniman,
Dirk Eggink,
Chantal Reusken,
Godelieve J. de Bree,
Rogier W. Sanders,
Marit J. van Gils,
Albert J. R. Heck

Affiliations

Danique M. H. van Rijswijck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht
Albert Bondt: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht
Max Hoek: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht
Karlijn van der Straten: Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity
Tom G. Caniels: Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity
Meliawati Poniman: Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity
Dirk Eggink: National Institute for Public Health and the Environment, RIVM
Chantal Reusken: National Institute for Public Health and the Environment, RIVM
Godelieve J. de Bree: Department of Internal Medicine, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Institute for Infection and Immunity
Rogier W. Sanders: Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity
Marit J. van Gils: Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity
Albert J. R. Heck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht

DOI: https://doi.org/10.1038/s41467-022-33899-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Profiling antibody repertoires using proteomic approaches may provide a way of screening for antibody cross-reactivity against SARS-CoV-2 variants. Here the authors use a IgG1 specific cleavage method to analyse the IgG1 repertoire within recovered patients and relate this to antibody binding and neutralisation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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